Ongoing Research Overview

The research and development at Nolla Antimicrobial is two-tiered. The company has studies running on classified and unclassified basis. Target of the unclassified research is academic publications, while classified research aims to strengthen patent rights.

Unclassified research

Unclassified studies initiated by Nolla Antimicrobial in collaboration with universities and research organizations.

1) Structure and characterization of novel Polyethyleneimine-Silver Chloride ionomer
The ionic bonding between silver ion and polyethyleneimine’s amine functionality can be qualified by IR and NMR spectroscopy. Interpretation of IR transmittance and NMR spectra can be verified by TEM imaging. In addition, TEM imaging reveals even distribution of silver ions within self-organized amine functional polymer.

2) Effects of Polyethyleneimine-Silver Chloride ionomer on cytotoxicity and cell irritancy in viable skin
Potential skin irritation, cell irritancy and cytotoxicity are investigated in natural and viable skin using the ex vivo Bovine Udder System (BUS) model. Purified water diluted Polyethyleneimine-Silver Chloride ionomer structures are openly applied to the skin. Full-thickness skin biopsies are taken from treated as well as from untreated skin after three exposure times (30 min, 1 h and 5 h). Inflammatory and cytotoxic potential is then biochemically analyzed to determine the skin compatibility of the compound. Cell irritancy value of the compound is evaluated by measuring concentration of specific proinflammatory mediator prostaglandin in full-thickness skin biopsies. Cytotoxic potential is determined in the MTT test by measuring the inactivation of mitochondria. The first results have demonstrated excellent skin compatibility of Polyethyleneimine-Silver Chloride inducing lower values for Polyethyleneimine-Silver Chloride ionomer compared to traditional alcohol-based disinfectants.

3) Polyethyleneimine-Silver Chloride ionomer coated cotton and viscose fibers
The well-known antimicrobial efficacy of silver is optimized by maximizing the surface area of silver on the fiber surface. Therefore, silver has been incorporated in fibers in the form of nanosilver for higher efficacy. By using a polymeric binder for silver ions, we hypothesize that the higher antimicrobial activity of silver is achieved compared to traditional methodologies such as nanocoating. In addition, very good adhesion is obtained by using polyethyleneimine, known for its characteristic adhesive properties. Cotton and viscose fibers were coated with Polyethyleneimine-Silver chloride ionomer in laboratory scale wet-coating process. Several concentrations of silver were used. Quantitative analysis of antimicrobial efficacy of the fibers were evaluated by using an industry-standard AATCC protocols. Preliminary results has shown excellent antimicrobial efficacy against both gram-positive and gram-negative strains.

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